Antibody Engineering: A Practical Guide (Breakthroughs in Molecular Biology)

At present, antibody engineering is an exploding field, with a rapid increase in the number of research scientists and laboratories involved. Due to this rapid growth, few laboratories entering the field possess the necessary expertise to set up new research programs. Antibody Engineering: A Practical Guide addresses this problem. Using state-of-the-art technologies, it presents an overview of antibody engineering for the production of recombinant human or mouse monoclonal antibodies. Among the topics that are thoroughly explored are antibody structure relevant to antibody engineering, plant and mammalian expression vectors and hosts, recombinatorial cDNA libraries, PCR cloning of single cells, and engineering of affinity and biological effector functions. Each chapter focuses on a particular aspect of antibody engineering and is written by a leading expert in the field.