Antisense Technology: A Practical Approach (Practical Approach Series)

0199635838. Softcover, colour fade on spine, from closed pharmaceutical company library. 326 pages, index, illustrated with charts, tables and diagrams. Step by step protocols for antisense techniques in a range of different organisms and cell culture systems. Discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation, and use of antisense oligonucleotides; in vitro R.N.A. transcription; construction strategies, testing and optimization of catalytic antisense R.N.As based on hammerhead ribozymes; synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of R.N.A. The numerous hints and tips for success and advice on trouble shooting, make this text invaluable to all those researchers who work on gene expression, R.N.A. transcription, or protein function. Contents clean, tight and bright.…