Self - Assembly Of Thin Films For Localized Gene Delivery Systems

Language: English

Published by LAP LAMBERT Academic Publishing Sep 2010, 2010

3838323823 / 9783838323824

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This item is printed on demand - Print on Demand Titel. Neuware -Gene delivery from reducible layer-by-layer (LbL) films has shown to be an effective way of delivering DNA both spatially and temporally. By incorporating DNA as the polyanion in the system, the polycation can be altered in order to trigger the release of DNA from bioreducible LbL films. This thesis focuses on the biological aspects of bioreducible LbL films, the effect of altering the mechanical behavior of LbL films with regards to cell adhesion and transfection activity, as well as the release dynamics of DNA from bioreducible LbL films. It has been found that bioreducible LbL films have high transfection activity in vitro and sustained levels of transfection in vivo. Futhermore, it was found that bioreducible films are biocompatible in vivo. Studies also found that the rigidity of the films can be increased via cross-linking LbL films or by simply adjusting the ionic strength of the deposition solution. In vitro studies showed that the increase in rigidity also increases the transfection activity of cells. Finally, atomic force microscopy (AFM) experiments were performed to investigate DNA release from bioreducible LbL films.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 204 pp. Englisch.…

Seller Inventory # 9783838323824

Title
Self - Assembly Of Thin Films For Localized Gene Delivery Systems
Author
Jenifer Blacklock
Publisher
LAP LAMBERT Academic Publishing Sep 2010
Publication year
2010
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3838323823
ISBN 13
9783838323824
Item weight
322 grams
Dimensions
220x150x13 mm

buchversandmimpf2000

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