This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.

About the Author

Jan Michiels Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, KU Leuven - University of Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Phone: +32 16 32 16 31 (secretariat) or +32 16 32 96 84 (office) Fax.: +32 16 32 19 63 Email: jan.michiels@biw.kuleuven.be Maarten Fauvart Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, KU Leuven - University of Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Tel: +32 16 32 16 31 (secretariat) or +32 16 32 96 80 (office); Fax: +32 16 32 19 63 Email: maarten.fauvart@biw.kuleuven.be

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