Basic Cell Culture (Paperback)

Davis

ISBN 10: 0199638535 / ISBN 13: 9780199638536

Published by Oxford University Press, Oxford, 2002

About this Item

Paperback. The use of animal, including human, cell culture has expanded enormously during the last 25 years, with new applications appearing all the time. The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basictechniques.Revised and updated chapters cover topics such as equipping a cell culture laboratory, sterilization, culture media, basic culture technique, cell cloning, the isolation of new cell lines,contamination procedures, and good laboratory practice. There is a brand new chapter on the microscopy of living cells, and an appendix of online resources for cell biology. This book is a guide for the newcomer to all those areas which are currently basic to the performance of animal (including human) cell culture. Experienced workers should also find it useful as a source of reference when entering a new field, or when setting up a new laboratory. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Seller Inventory # 9780199638536

Bibliographic Details

• Title: Basic Cell Culture (Paperback)
• Publisher: Oxford University Press, Oxford
• Publication Date: 2002
• Language: English
• ISBN 10: 0199638535
• ISBN 13: 9780199638536
• Binding: Paperback
• Edition: 2nd Edition
• Condition: new

About this title

Synopsis

The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.

About the Author

John Davis is at Bio-Products Laboratory, Elstree, Hertfordshire, UK.