Basic Cloning Techniques: A Manual of Experimental Procedures

204 pages. Ex-library This manual is written by the staff of the School of Biological Sciences at the University of Leicester, and based on the successful cloning courses held there. It provides a practical guide, with theoretical background, through the maze of complex techniques now available for undergraduates and postgraduates and resea rch workers interested in prokaryote and eukaryote molecular biology, with the a im of making these techniques more accessible. It provides a detailed and cohere nt recipe-type guide to complex experiments with comparative discussion of the m erits of the different techniques. The emphasis reflects precise experimental pr otocols developed at Leicester over several years. Where possible the details an d source of the various strains used are listed, and recipes are supplied in the appendices.