Basic HPLC and CE of Biomolecules

This text provides explanations and examples of the primary chromatographic techniques for biomolecules (reversed-phase, size exclusion, ion-exchange, hydrophobic interaction and affinity). It also offers practical suggestions for their implementation and optimization. An extensive bibliography furnishes a wealth of background information.

Robert L. Cunico holds degrees in chemistry from Colorado State University and San Francisco State University, where he also did postgraduate work. At Varian Associates, he developed HPLC methods, columns and instrumentation for biomolecules and served as the HPLC training manager. Bob then joined the analytical group at Cetus Corporation (now Chiron) where he developed and validated methods for protein therapeutics. In 1991, he founded Bay Bioanalytical Laboratory, Inc., a consulting and contract laboratory serving the biotechnology and biopharmaceutical industry. As president and principal scientist of BBL, Bob has been a contributor to numerous INDs. Karen M. Gooding began her career in the laboratory of Fred Regnier at Purdue University, participating in the original development of columns and techniques for HPLC of proteins. In 1977, Karen and David Gooding founded SynChrom, Inc. specifically for the purpose of developing and manufacturing HPLC columns for protein analysis. As Analytical Director and President of SynChrom, Karen guided efforts in methods development with the express goal of expediting protein analysis. She has published extensively in the chromatography field and has served as an editor of Journal of Chromatography, Trends in Analytical Chemistry, and, with Fred Regnier, of the book, HPLC of Biological Macromolecules: Methods and Applications. Tim Wehr received his Ph.D. in microbial physiology at Oregon State University and did postdoctoral research in molecular biology at UC Berkeley. He managed the HPLC applications lab at Varian Associates for eight years and worked on development of LC columns and HPLC-based analyzers. For the last eight years he directed the CE chemistry R&D group at Bio-Rad Laboratories, developing CE instrumentation, methods, and application kits. He has published extensively in the separation sciences, and served for nine years on the organizing committee of the International Symposium on HPLC of Proteins, Peptides and Polynucleotides.