Bio-analytical method development of antimalarial drug in human plasma | Validation for quantitative estimation by using LC-MSMS

Bincy Abraham (u. a.)

ISBN 10: 6200482470 / ISBN 13: 9786200482471

Published by LAP LAMBERT Academic Publishing, 2019

About this Item

Bio-analytical method development of antimalarial drug in human plasma | Validation for quantitative estimation by using LC-MSMS | Bincy Abraham (u. a.) | Taschenbuch | Englisch | 2019 | LAP LAMBERT Academic Publishing | EAN 9786200482471 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.

Seller Inventory # 120469922

Bibliographic Details

• Title: Bio-analytical method development of antimalarial drug in human plasma | Validation for quantitative estimation by using LC-MSMS
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2019
• Language: English
• ISBN 10: 6200482470
• ISBN 13: 9786200482471
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 220W x 150H x 9D millimeters 220 millimeters width by 150 millimeters height by 9 millimeters depth
• Weight: 209 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

In the present work new, simple reversed-phase LCMS/MS in positive ion mode method was developed and validated for the determination of an anti-malarial in blood plasma. Drug was used as an internal standard. The chromatographic separation was achieved with Zorbax SB-C18, 5.0µm (150*4.6mm). The separation of Drug H was achieved by gradient elution using organic mixture (Acetonitrile: Methanol::80:20 v/v), buffer solution (10mM Ammonium Formate) and DMSO:: 50:50:0.2 v/v/v. The flow rate is 1.000 ml/min, Injection Volume of 10µl with a run time of 3.0 minutes. The calibration curve of standard Drug H was linear in range 0.1-20.0 μg/ml. 5 precision and accuracy batches were run; the coefficient of correlation (r2) was ≥ 0.99. The accuracy of the LLOQ in the standard CC was within ± 20% of the nominal concentration and ± 15% of the nominal concentration for other standards and 75% non- zero calibration standards met the acceptable criteria which indicated the method was precise and accurate. Method validation results have proved the method to be selective, sensitive, precise, accurate and robustness.