Biocomputing and biochemical studies of enzymes : Application in Tissue Kallikreins and Metalloproteases

Language: English

Published by VDM Verlag Dr. Müller, 2011

3639185900 / 9783639185904

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Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH

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nach der Bestellung gedruckt Neuware - Printed after ordering - The human kallikrein family is a family of proteolytic enzymes, classified as serine proteases, that derive from chromosome 19, locus 13.3-13.4. One member of the family, human kallikrein 4 (hK4) which is thought to carry out crucial functions in the prostate, was expressed in this study as a secreted protein in a baculovirus expression system, bearing a His-tag and V5-epitope that were used for purification and detection respectively. Computational analysis was included by applying high-performing computing techniques, such as molecular dynamics, and flexible ligand docking, to predict antigenic regions, the likely substrate specificity and putative inhibitors. These results show that hK4 has a loop, between Leu83-Ser94 that shows promise as a specific segment that can be exploited for generation of antibodies. The principles of substrate-specificity analysis that were developed were further applied on three metzincins, MMP-3, ADAM-9 and ADAM-10. These three enzymes are metalloproteases, which are involved in tissue remodeling, intracellular signalling and cell-to-cell mediation. This study is a demostration of the power of combining bioinformatics + wet-lab biochemistry. …

Seller Inventory # 9783639185904

Title
Biocomputing and biochemical studies of enzymes : Application in Tissue Kallikreins and Metalloproteases
Author
Sergio Manzetti
Publisher
VDM Verlag Dr. Müller
Publication year
2011
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3639185900
ISBN 13
9783639185904
Item weight
320 grams
Dimensions
220x176x33 mm

AHA-BUCH GmbH

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