Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo

Thomas Harry Sharp

ISBN 10: 3319021583 / ISBN 13: 9783319021584

Published by Springer International Publishing Nov 2013, 2013

About this Item

This item is printed on demand - it takes 3-4 days longer - Neuware -As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis. 168 pp. Englisch.

Seller Inventory # 9783319021584

Bibliographic Details

• Title: Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo
• Publisher: Springer International Publishing Nov 2013
• Publication Date: 2013
• Language: English
• ISBN 10: 3319021583
• ISBN 13: 9783319021584
• Binding: Buch
• Condition: Neu
• Dimensions: 241W x 160H x 15D millimeters 241 millimeters width by 160 millimeters height by 15 millimeters depth
• Weight: 424 grams

About this title

Synopsis

As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.

About the Author

Thom Sharp completed his bachelor’s degree in Biochemistry at the University of Bristol, with a year spent a year working at AstraZeneca. He continued in Bristol for his PhD in bionanotechnology, which was performed under the supervision of Dek Woolfson, a protein design chemist, and Paul Verkade, an electron microscopist. Thom is currently at the University of Oxford, where he is developing DNA-templated protein arrays to increase the throughput of single-particle analysis.