This Reprint contains eleven papers published in the Special issue of the International Journal of Molecular Sciences entitled "Biomolecular Structure, Function and Interactions". These papers bring together examples of various experimental, computational, and machine-learning methodologies in biophysical chemistry. Biophysical chemistry is a truly interdisciplinary research area combining the principles of physics, biology, and chemistry to explore the processes in biological systems and their underlying physical and chemical properties. The focus of the researchers in this field is on understanding how biological molecules, such as proteins, nucleic acids, and lipids, interact with each other and with their environment at the molecular level. Thus, biomolecular interactions are a fundamental part of biophysical chemistry. Biophysical chemists are also occupied with the determination of the structure and function of biomacromolecules.

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