Paperback. Pub Date: 2015-04-01 Pages: 153 Language: Chinese Publisher: China Agriculture Press Biotechnology experimental guidance comprehensive. systematic optimization screened discipline * Basic experiment content. and on this basis. design We launched a comprehensive. innovative big experiment; focus on students basic experimental skills development while more enhanced the overall quality of students and the ability to enhance the students' independence to carry out experiments. scientif.

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Bibliographic Details

Title

Biotechnology experimental guidance(Chinese Edition)

ISBN 10

7109203735

ISBN 13

9787109203730

Binding

paperback

Condition

New

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Synopsis

《生物技术实验指导》全面、系统地优化筛选了本学科*基本的实验内容，并在此基础上，设计推出了综合性、创新性大实验；注重对学生进行基本实验技能培养的同时，更加强化了学生综合素质与能力的培养，加强了对学生独立开展实验、科学思维的产生与提升、方法与技术集成创新的意识、勇于实践探索精神等个性特质的挖掘、锻炼和塑造，给予学生更多参与实验的机会。《生物技术实验指导》可以作为高等院校生物技术相关课程的实验课教材，也可作为研究生、有关科技人员的参考书。《生物技术实验指导》在高等院校的推广使用，必将惠及广大学生，推动生物技术实验教学质量的提高，促进人才培养的快速发展。前言第1章细胞生物学1-1暗视野显微镜的原理及使用1-2相差光学显微镜的原理及使用1-3透射电子显微镜的原理及使用1-4扫描电子显微镜的原理及使用1-5细胞膜的渗透性1-6细胞的凝集反应1-7聚乙二醇法诱导细胞融合1-8线粒体和液泡系的超活染色1-9叶绿体和细胞核的荧光观察1-10细胞骨架的观察1-11DNA的细胞化学——Feulgen反应1-12RNA的细胞化学——Brachet反应第2章细胞工程2-1MS培养基母液的制备2-2MS培养基的配制2-3烟草无菌接种技术2-4愈伤组织的增殖培养2-5芽的诱导培养2-6根的诱导培养2-7组织培养苗的移栽2-8植物原生质体融合2-9叶绿体的分离与观察2-10大蒜生长点的剥离与接种2-11植物细胞的生长计量技术2-12马铃薯茎尖培养脱毒2-13无菌实生苗的培育2-14植物病毒机械传染技术2-15植物原生质体超薄切片2-16原生质体的扫描电子显微镜制样2-17植物病毒样品负染色技术第3章基因工程3-1琼脂糖凝胶电泳技术3-2植物总DNA的提取3-3植物总RNA的提取3-4反转录PCR克隆目的基因3-5外源DNA片段与质粒载体的重组连接3-6感受态细胞的制备及质粒DNA的转化3-7质粒DNA的提取3-8外源基因在大肠杆菌中的表达及其检测3-9农杆菌介导的植物遗传转化3-10基因枪介导的植物遗传转化3-11转基因植物的Southem杂交分析3-12转基因植物的Northern杂交分析3-13转基因植物的western杂交分析3-14酶联免疫吸附技术第4章分子免疫学4-1免疫血清的制备4-2免疫扩散法检测抗体的效价4-3免疫电泳技术4-4T淋巴细胞和B淋巴细胞的分离技术4-5细胞因子检测技术4-6免疫荧光技术4-7酶联免疫吸附测定技术4-8生物素一亲和素免疫标记技术第5章现代仪器分析5-1维生素C标准曲线的制作及浓度对朗伯-比尔定律偏离的影响5-2火焰原子吸收光谱法测定水中钙的含量——标准加入法5-3气相色谱分离效能指标的测量5-4果蔬中抗坏血酸的高效液相色谱分析5-5果蔬乙烯释放量的气相色谱分析第6章常用仪器设备及实验技术6-1常用仪器设备及用途6-2洗涤技术6-3灭菌技术6-4无菌操作技术6-5药品配制技术6-6其他实验技术第7章实验室安全管理7-1概述7-2实验室的安全制度及应急预案7-3实验操作及实验仪器运行安全附录常用溶液的配制主要参考文献

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