Paperback Pages Number: 268 Language: Chinese. Biotechnology experiments the basic order in accordance with the biotechnology process followed by choreography. covering species (including algae. cell lines) breeding. fermentation and cell culture. bioseparations biotechnology aspects of the experimental projects. More abundant. both basic experiment. and experiment. as well as research. design of experiments. Part of the experimental project is Editor in carrying out preliminary research proj.

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Bibliographic Details

Title: Biotechnology experiments(Chinese Edition)
Publisher: China Press
Publication Date: 2000
Language: English
ISBN 10: 7305096903
ISBN 13: 9787305096907
Binding: paperback
Condition: New

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Synopsis

《生物工艺实验》基本按照生物工艺全过程的顺序依次编排，涵盖了菌种（包括藻种、细胞株等）选育、发酵及细胞培养、生物分离等生物工艺环节的实验项目。内容较为丰富，既有基础性实验，又有综合性实验，还有研究、设计性实验。部分实验项目是编者在开展“973”前期研究专项、国家科技支撑计划课题等研究工作中提炼出来的局部阶段性成果。因此，《生物工艺实验》应该是生物工程等相关专业本科学生开展专业实验的合适指导书，也是生物化工等相关专业研究生开展实验研究的合适参考书。第一章生物工艺实验基础知识第一节生物工艺实验规则与安全守则第二节实验方案的设计与实施第三节实验数据的记录和处理第四节生物工艺实验基本操作实验一常用玻璃仪器及其清洗干燥实验二培养基的配制及灭菌第二章茵／藻种选育及保藏工艺实验第一节自然选育实验实验一螺旋藻藻种的分离筛选实验二α一淀粉酶产生茵的分离筛选实验三高产淀粉酶菌株复筛实验四富油微藻的分离筛选第二节诱变育种实验实验五紫外线诱变育种实验六亚硝基胍诱变育种实验七抗结构类似物菌株的筛选实验八营养缺陷型菌株的筛选第三节基因工程育种实验实验九质粒DNA的小量制备实验十细菌的局限性转导实验十一基因工程菌株的培养与观察第四节菌种保藏实验实验十二菌种常规保藏实验十三菌种冷冻干燥保藏第三章发酵工艺实验第一节液体静置发酵实验一啤酒酵母的质量检查实验二啤酒酵母的计数实验三糖度测定实验四啤酒酸度和pH的测定实验五σ氨基氮的测定实验六双乙酰含量的测定实验七苦味质含量的测定实验八啤酒酵母的扩大培养及干酵母活化实验实验九100L小型啤酒酿造设备介绍及酿造设备CIP消毒实验十100L小型啤酒酿造实验--麦汁制备实验十一100L小型啤酒酿造实验--主发酵实验十二100L小型啤酒酿造实验--后发酵实验十三酒精度的测量及原麦汁浓度实验十四啤酒质量品评实验十五啤酒固定化发酵实验十六啤酒加压发酵实验十七固定化酵母发酵纤维素制酒精第二节深层液体通风发酵实验实验十八谷氨酸及还原糖含量测定实验十九镜检及OD检测实验实验二十发酵罐的构造及灭菌实验二十一谷氨酸生产菌种的扩大培养实验二十二谷氨酸的中糖发酵实验二十三谷氨酸的等电回收第三节固态发酵实验二十四白酒中总酸、挥发酸、非挥发酸的测定实验二十五白酒中总酯的测定实验二十六白酒中甲醇的含量分析实验二十七小曲酒发酵实验实验二十八半固态白酒蒸馏实验二十九固体曲的制备第四章细胞培养工艺实验第一节动物细胞培养实验一小鼠肺组织细胞原代培养实验二小鼠肺组织细胞传代培养实验三人外周血淋巴细胞悬浮培养及染色体制片实验四Marc145细胞的微载体培养第二节植物细胞培养实验五胡萝卜储藏根愈伤组织培养实验六香蕉吸芽组织培养实验七胡萝卜愈伤组织细胞悬浮培养……第五章生物高工艺实验附录参考文献

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