Cardiac Regeneration: Methods and Protocols (Methods in Molecular Biology)

This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.