Cell-Free Gene Expression : Methods and Protocols

Michael C. Jewett

ISBN 10: 1071619977 / ISBN 13: 9781071619971

Published by Springer US, Springer US, 2022

About this Item

Druck auf Anfrage Neuware - Printed after ordering - This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.

Seller Inventory # 9781071619971

Bibliographic Details

• Title: Cell-Free Gene Expression : Methods and Protocols
• Publisher: Springer US, Springer US
• Publication Date: 2022
• Language: English
• ISBN 10: 1071619977
• ISBN 13: 9781071619971
• Binding: Buch
• Condition: Neu
• Dimensions: 260W x 183H x 30D millimeters 260 millimeters width by 183 millimeters height by 30 millimeters depth
• Weight: 1048 grams

About this title

Synopsis

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.