This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates; alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians and animal care technicians involved with reprogramming, nuclear transfer and transgenesis.

About the Author

Professor Paul J. Verma PhD Principal Scientist- Reproductive Biology South Australian Research Institute (SARDI) Adjunct Professor & Head Cell Reprogramming & Stem Cells Monash Institute of Medical Research 27-31 Wright Street Clayton, VIC 3168 AUSTRALIA Voice: + 61 3 9902 4771 Fax: + 61 3 9594 7416 Cell: +61 413 387 563 Email: Paul.Verma@sa.gov.au Email: Paul.Verma@monash.edu Huseyin Sumer Monash Institute of Medical Research Faculty of Medicine, Nursing and Health Sciences MIMR Centre for Reproduction and Development Phone: 61 2 990 24739 email: huseyin.sumer@monash.edu

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