Cell and Tissue Engineering of Cartilage | Regulation of Chondrocyte by TGF-� and ECM-Proteins

Seyed Ali Khaghani

ISBN 10: 3659113069 ISBN 13: 9783659113062

Published by LAP LAMBERT Academic Publishing, 2012

Language: English

Condition: New Soft cover

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Bibliographic Details

Title

Cell and Tissue Engineering of Cartilage | Regulation of Chondrocyte by TGF-� and ECM-Proteins

Publisher

LAP LAMBERT Academic Publishing

Publication Date

2012

Language

English

ISBN 10

3659113069

ISBN 13

9783659113062

Binding

Taschenbuch

Condition

Neu

Dimensions

Weight

334 grams

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Synopsis

Cartilage cell (Chondrocyte) is embedded in the matrix (Lacunae) and has round shape in vivo. The in vitro monolayer culture of primary chondrocyte causes morphological change characterized as dedifferentiation. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. The effect of TGF-β1, 2, 3, and their manipulated forms in biological regulation of primary chondrocyte was investigated in this work. A novel method was developed to isolate and purify the primary chondrocytes from knee joint of neonate Sprague-Dawley rat, and the effect of some supplementations such as hyaluronic acid and antibiotics were also investigated to provide the most appropriate condition for in vitro culture of chondrocyte cells.

About the Author

SA (Behruz) Khaghani, PhD (TE): Studied industry, mechanical and medical engineering in Turkey, Germany and United Kingdom respectively. He received PhD in cell and tissue engineering from University of Bradford and worked in industrial and large organisations for more than 18 years. He is currently a research fellow in cell and tissue engineering.

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