Cell adhesion behavior on molecularly engineered surfaces: Mechanisms of Cell/Surface Interaction

Cai, Ning

ISBN 10: 365919039X / ISBN 13: 9783659190391

Published by LAP LAMBERT Academic Publishing, 2012

About this Item

Seller Inventory # 26357452893

Bibliographic Details

• Title: Cell adhesion behavior on molecularly engineered surfaces: Mechanisms of Cell/Surface Interaction
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2012
• Language: English
• ISBN 10: 365919039X
• ISBN 13: 9783659190391
• Binding: Soft cover
• Condition: New

About this title

Synopsis

Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute. Better understanding of biophysical responses of adherent cells is of great importance in optimizing biomaterial surface for tissue engineering applications. In this work, poly(lactic acid) (PLA) and carbon nanotubes (CNT), were surface-modified with extracellular matrix (ECM) proteins to improve their compatibility. The results of adhesion dynamics of porcine esophageal fibroblasts (PEFs) on ECM protein-immobilized PLA and CNT monolayer demonstrate the different biophysical responses of PEFs on unmodified and ECM protein-modified biomaterial surfaces. ECM protein immobilization effectively promotes adhesion of PEFs to biomaterials surfaces. In addition, it is found that functional blocking of β1 integrin impaires cell-fibronectin interactions. Adhesion strength is tightly correlated to focal adhesion density, which is explained by peeling and fracture models. Furthermore, Weibull distribution is proved to satisfactorily describe cell adhesion strength in adherent cell population.

About the Author

Dr. Ning Cai received Ph.D. degree in Biomedical Engineering from Nanyang Technological University, Singapore. He Joined Wuhan Institure of Technology (WIT) as WIT Scholar in 2011. Dr. Cai has published over 10 top quality international conference and journal papers. His research interests include biomaterials and tissue engineering.