The Centrosome and its Functions and Dysfunctions

Introduction

1) Cell and Molecular Biology of Centrosome Structure and Function

1.1 Historical Perspectives

1.2 Centrosome Structure, Molecular Composition, and

Function

1.3 Centrosome Matrix and Centrosomal Proteins

Centrosome Proteins

1.4 Centriole Structure and Cell Cycle-specific centriole

dynamics

1.5 In vivo imaging of Centrosomes

2) The centrosome Cycle within the cell cycle

2.1 Centrosome-nuclear interactions

2.2 Centrosome maturation, mitosis and cell division

this all needs sorting

2.3 Chromosome (DNA)-centrosome synchronization cycle - this all needs sorting

2.4 Centrosome-Golgi relationships and interconnected functions

2.5 Primary cilia

3) Centrosome as center for proteolytic activity and dysfunctions associated with pathogenesis of human disease

3.1 Centrosomes as center for proteolytic activity and

dysfunctions in disease

3.2 Other centrosome-related dysfunctions in disease

3.3 Vesicular transport dysfunctions and disease

3.4 Basal body dysfunctions and disease

4) Centrosome dysfunctions in cancer

Needs sorting

- Viral transformation and aneuploidy

- Oncogenes and tumor suppressor genes

- Centrosomes as targets for cancer therapy

5) Virus exploitation of centrosomes

6) Centrosomes in reproduction

Introduction

6.1 The importance of choosing an appropriate animal

model

6.2 Centrosome terminology questions in reproductive cells

6.3 Centrosome reduction during gametogenesis

6.4 The Sperm Centriole Complex Before Fertilization

6.5 Centrosomes in unfertilized eggs and oocytes

6.6 Centrosomes during oocyte maturation, fertilization

and first cell division

- oocyte maturation

- fertilization and first cell division

6.7 Effects of aging on oocyte centrosomes (something like

this)

7) Transition from centrosomal to non-centrosomal microtubule organization during cellular polarization

8) External and environmental effects on Centrosomes

8.1 Effects of ionizing radiation on centrosomes

8.2 Environmental and genotoxic stress on centrosomes

9) Centrosomes and centrosome equivalents in other systems

9.1 Stem cells

9.2 Neuronal cells

9.3 T cells (cytotoxic T-lymphocytes; CTLs)

9.4 Centrosomes and centrosome equivalents in other

systems and in model organisms

- Parasites

- Yeast (spindle pole bodies; SPBs)

- Dictyostelium (nuclear associated bodies; SPBs)

- C elegans

- Drosophila

10) Non-centrosomal microtubule organization in plant cells