Characterization of Groundnut Cultivars through Morphological and RAPD

Present investigation was carried out using15 genotypes of groundnut. The crop was sown during Kharif season and ten morphological characters were studied and further we isolated DNA of all, did random amplification using 15 RAPD primer. ANOVA revealed significant mean squares due to genotypes for all the characters thereby indicating substantial amount of genetic variability among the genotypes. Cluster analysis using Ward's method showed sufficient genetic distance among the gentypes. Purity of isolated DNA through CTAB method was analyzed through spectrophotometer found fairly good. Total 15 primers were screened, out of which 13 primers produced amplification. A total 15 primers were screened, out of which 13 primers produced amplication. A total 54 scorable bands were produced, out of which 28 bands were polymorphic and the level of polymorphism was 51.8 %. The band information is used to generate dendrogram using UPGMA software. The similarity coefficient ranged from 0.76 to 0.94. Dendrogram divide the 15 genotypes into six clustereds.