Chimeric RNA

Justin Elfman

ISBN 10: 1493999036 / ISBN 13: 9781493999033

Published by Springer US Nov 2019, 2019

About this Item

This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome. 276 pp. Englisch.

Seller Inventory # 9781493999033

Bibliographic Details

• Title: Chimeric RNA
• Publisher: Springer US Nov 2019
• Publication Date: 2019
• Language: English
• ISBN 10: 1493999036
• ISBN 13: 9781493999033
• Binding: Buch
• Condition: Neu
• Dimensions: 260W x 183H x 21D millimeters 260 millimeters width by 183 millimeters height by 21 millimeters depth
• Weight: 722 grams

About this title

Synopsis

This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome.