Cloning of Human Insulin Gene in Bacterial Expression System

This book is containing the genetic engineering methods for production of Human insulin in bacterial expression system, under Iraqi conditions and the study of its treatment application. The insulin gene was sub cloned into expression vector pET- Blue- 1 vector, and a new constructive vector pET- Blue- 1-hpi vector was achieved as a first time in the world with about 4659 kb., the transformation processes were done directly to three types of genetic engineering bacterial cells which are : Nova Blue Singles™, Tuner (DE3) pLacI and E. coli HB101.

Dr.Afrodet Abdul Razaq Salih has obtained her PhD. degree in Biotechnology in 2012 from Basrah University.She is a lecturer in College of Science, University of Basrah, Iraq.