DNA Cloning and Assembly Methods (Methods in Molecular Biology; Springer Protocols)

Svein Valla; Rahmi Lale

ISBN 10: 1627037632 / ISBN 13: 9781627037631

Published by Humana Press, 2014

About this Item

Oversized hardcover, xi + 303 pages, NOT ex-library. A number highlited on p.10; a pencil note on p.25 and p.36, short pencil notations on 4 other pages, some pencil underlining. Else good, mostly clean condition with unmarked text, free of inscriptions and stamps, firmly bound. Issued without a dust jacket.

Seller Inventory # 009221

Bibliographic Details

• Title: DNA Cloning and Assembly Methods (Methods in Molecular Biology; Springer Protocols)
• Publisher: Humana Press
• Publication Date: 2014
• Language: English
• ISBN 10: 1627037632
• ISBN 13: 9781627037631
• Binding: Hardcover
• Condition: Good

About this title

Synopsis

In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.

With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.