DNA Nanotechnology for Bioanalysis, From Hybrid DNA Nanostructures to Functional Devices

This book is intended for non-specialists and students, presenting a unique introduction to the field of DNA nanotechnology. The primary focus is on the extraordinary advantages of specificity and sensitivity obtained by integrating DNA nanostructures in bioanalytical devices.

DNA Nanotechnology for Bioanalysis provides a concise and rigorous description for the fabrication of various types of functional nanostructures by optimized software-aided high-yield synthesis. Following this is the explanation of methods to decorate these nanostructures with molecules such as proteins, metal nanoparticles or bioorganic moieties covalently bonded onto DNA via self-assemblage processes. Also provided is a concise review on non-canonical DNA structures (such as G-quadruplexes) and their targeting by small molecules for applications in pharmacology. Finally, it describes the exciting applications of DNA nanostructures in life sciences and nanomedicine, including ultraspecific molecular delivery, control of cell behavior, analysis of cell lysate and DNA-based nano-tools for super-resolution sub-cellular imaging.

Readership: Non-specialists, undergraduate students and researchers who are interested in learning the basics of DNA nanotechnology.

Giuseppe Arrabito received his PhD (2012) from Scuola Superiore di Catania. He carried out Post-Doctoral fellowships in the group of C M Niemeyer (2013) and in the group of C Falconi (2014). He is currently a Post-Doctoral Scientist in the group of B Pignataro. His main research interests are in the field of bioanalytical chemistry, micro- and nano-biological array fabrication, Dip Pen Nanolithography, DNA directed material assembly for nanotechnological applications, and ZnO nanostructures for biosensing.

Liqian Wang received her PhD degree (2016) in Materials Science from the University of Rome Tor Vergata. She worked as a visiting researcher (2014) in the group of Ilia N. Ivanov at Oak Ridge National Laboratory. She is currently a Post-Doctoral Scientist in the group of Chunhai Fan. Her research interests include the application of DNA nanostructures as nanoelectronic devices and the interface between organic self-assembly and inorganic nanotechnology.