The use of light for probing and imaging biomedical media is promising for the development of safe, noninvasive, and inexpensive clinical imaging modalities with diagnostic ability. The advent of ultrafast lasers has enabled applications of nonlinear optical processes, which allow deeper imaging in biological tissues with higher spatial resolution. This book provides an overview of emerging novel optical imaging techniques, Gaussian beam optics, light scattering, nonlinear optics, and nonlinear optical tomography of tissues and cells. It consists of pioneering works that employ different linear and nonlinear optical imaging techniques for deep tissue imaging, including the new applications of single- and multiphoton excitation fluorescence, Raman scattering, resonance Raman spectroscopy, second harmonic generation, stimulated Raman scattering gain and loss, coherent anti-Stokes Raman spectroscopy, and near-infrared and mid-infrared supercontinuum spectroscopy. The book is a comprehensive reference of emerging deep tissue imaging techniques for researchers and students working in various disciplines.

About the Author

Lingyan Shi is an Assistant Professor in the Department of Bioengineering at the UC San Diego Jacobs School of Engineering. She is developing/applying new optical imaging and spectroscopic technologies to solve biomedical questions. Her recent major scientific contributions include the discovery of the "Golden Optical Window" for deep brain imaging, and the development of a breakthrough platform that combines deuterium probing with Stimulated Raman Scattering (SRS) for imaging of metabolic dynamics in living organisms. Shi is a member of Tau Beta Pi, OSA, SPIE, SfN, ASME, NYAS, and BMES.

Robert R. Alfano is a distinguished professor of science and engineering at the City College of New York. He has pioneered many applications of light and photonics technology to study biological, biomedical, and condensed matter systems using optical spectroscopy and imaging. He discovered and has used supercontinuum. Prof. Alfano is a fellow of the American Physical Society, the Optical Society, and the Institute of Electrical and Electronics Engineers.

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