Design, Synthesis and Cloning of Adult Human Hemoglobin Gene

This book describes Dr. Agarwal's work on development of recombination adult human hemoglobin to be utilized for artificial blood substitutes at the LA Tech University. The work was conducted with able guidance from Dr. Donald Haynie, PhD and Dr. Steven Jones, PhD within the Department of Biomedical Engineering. This book mainly elaborates on the molecular biology techniques like gene design and synthesis employing Polymerase Chain Reaction, cloning and protein expression of Adult Human Hemoglobin to be carried in bio-compatible nano-spheres using Layer by Layer Assembly, to function as a artificial blood substitutes.

Dr. Agarwal is staff physician at Methodist Charlton Medical Center, Medical Director for Redbird Healthcare Center,Dallas,Texas,USA. He is trained in Family Medicine,Nuclear Medicine,American Board of Nuclear Medicine certified,and has a PhD in Biomedical Engineering. Dr. Jones is professor of Biomedical Engineering at LA Tech University, USA.