How to Determine the Structure and Function of Glycosyl Hydrolases and Trehalases in Mycobacterium Tuberculosis and Its Role in Virulence.

Reports documented in biomedical literature indicates that Glycosyl hydrolases and trehalases in Mycobacteria especially in Mycobacterium tuberculosis may play a role in its virulence. Enzymatic assays are required to see if one can detect specific enzyme activity in M. tuberculosis through microbiological techniques, protein expression and purification, crystallization experiments, glycosyl hydrolase and trehalase assays and gene cloning which will help to determine the role of the enzyme in virulence. It will also enable us to identify the outer membrane protein required for the transport of lipopolysaccharides to the bacterial cell surface.