Development and Validation Method For Polyherbal Marketed Formulation: Using HPTLC and RP-UHPLC

Bacopa monnieri is a nootropic herb distributed throughout the warm wetlands of the world. B. monnieri has various medicinal properties, and these medicinal aspects are discussed in several reviews. This article is envisaged in the context of neuroprotection by this herb and its major active constituents. Ancient Vedic scholars frequently used B. monnieri to memorize lengthy sacred hymns and scriptures. In India, in Ayurvedic prescriptions, B. monnieri has been consumed as ‘medhyarasayana’ (in Sanskrit, ‘medhya’ -intellect or cognition, ‘rasayana’ - rejuvenation). Many Ayurvedic preparations prescribed for cognitive dysfunction contain B. monnieri as a prime constituent. In Charaka Samhita (6th century AD), B. monnieri is mentioned as a medicine for the management of mental dysfunctions such as anxiety, poor cognition, and lack of concentration. B. monnieri produces various metabolites such as saponins, alkaloids and sterols. The main active constituents of B. monnieri are dammarane-type triterpenoid saponins known as bacosides with jujubogenin or pseudo jujubogenin as their aglycone units. Bacosides are known for their nootropic and various other biological activities.