Digital Holography Microscopy applications : Three Dimensional Object Analysis and Tracking

Cedric Schockaert

ISBN 10: 3639136969 / ISBN 13: 9783639136968

Published by VDM Verlag Dr. Müller, 2009

About this Item

nach der Bestellung gedruckt Neuware - Printed after ordering - This book describes general and robust algorithms that are devoted to automate the analysis process in the 3D space and in time of dynamic objects present in a volume studied by a specific microscope that permits to record images containing their 3D information. This imaging system using a coherent light records not only the intensity information of the light, but also the direction of propagation for each pixel in the image. Together with a numerical model for coherent light diffraction, it is possible to generate numerically other images parallel to the recorded plane and as a consequence to refocus numerically objects. The acquisition time of 3D data is as a consequence only limited by the acquisition rate of the CCD camera of in the imaging system. The extraction of 3D information is performed by algorithm applied on the recorded data. The studied objects are either of biological nature or latex particles. A focus criterion to determine the best focus plane of an object is proposed. From the knowledge of the 3D position of objects, several analyses are proposed like shape or object trajectories analysis in time and in 3D.

Seller Inventory # 9783639136968

Bibliographic Details

• Title: Digital Holography Microscopy applications : Three Dimensional Object Analysis and Tracking
• Publisher: VDM Verlag Dr. Müller
• Publication Date: 2009
• Language: English
• ISBN 10: 3639136969
• ISBN 13: 9783639136968
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 220W x 150H x 11D millimeters 220 millimeters width by 150 millimeters height by 11 millimeters depth
• Weight: 287 grams

About this title

Synopsis

This book describes general and robust algorithms that are devoted to automate the analysis process in the 3D space and in time of dynamic objects present in a volume studied by a specific microscope that permits to record images containing their 3D information. This imaging system using a coherent light records not only the intensity information of the light, but also the direction of propagation for each pixel in the image. Together with a numerical model for coherent light diffraction, it is possible to generate numerically other images parallel to the recorded plane and as a consequence to refocus numerically objects. The acquisition time of 3D data is as a consequence only limited by the acquisition rate of the CCD camera of in the imaging system. The extraction of 3D information is performed by algorithm applied on the recorded data. The studied objects are either of biological nature or latex particles. A focus criterion to determine the best focus plane of an object is proposed. From the knowledge of the 3D position of objects, several analyses are proposed like shape or object trajectories analysis in time and in 3D.

About the Author

? 1997 - 2002: electrical civil engineer Université Catholique de Louvain with honors. ? 2004 - 2005: specialized study in image processing Université Libre de Bruxelles (ULB) with the highest honors. ? 2004 - 2007: (ULB) PhD thesis in image processing (digital holography) and optics for the European Space Agency.