Title: Digital Microscopy, Volume 81, Third Edition: Methods in Cell Biology (Volume 114)
Publisher: Academic Press
Publication Date: 2007
Language: English
ISBN 10: 0123740258
ISBN 13: 9780123740250
Binding: hardcover
Edition: 3rd Edition
Condition: New
Weight: 44 ounces

About this title

Synopsis

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.

Expands coverage to include discussion of confocal microscopy not found in the previous edition
Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

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