Ecto-Atpases : Recent Progress on Structure and Function

Liselotte Plesner

ISBN 10: 1461377293 / ISBN 13: 9781461377290

Published by Springer Us, 2012

About this Item

Druck auf Anfrage Neuware - Printed after ordering - It has been known for almost 50 years that many cells carry enzymes that hydro lyze extracellular ATP, and the term 'ecto-ATPase' was used first by Engelhardt 40 years ago. But until the end of the 1970's, the idea of an ATPase with its ATP hydrolyzing site on the outside of the cell membrane was met with substantial skepticism since it was thought that ATP was strictly intracellular. Nevertheless, ecto-ATPase activity was dem onstrated using a variety of intact cells. Most ecto-ATPase(s) exhibited three common 2 characteristics: 1) activation by either Ca + or Mg2+, 2) insensitivity to the commonly used inhibitors ofF-type, P-type, and V-type ATPases, and 3) ability to hydrolyze nucleo side triphosphates and often nucleoside diphosphates as well. At the same time, the dominant ATPase activity in many plasma membrane preparations was shown to be dis tinct from the ion-pump ATPases, but had similar enzymatic properties as the ecto-AT Pase(s). Thus the term 'E-type ATPase activity' has been proposed for ATPase activity exhibiting these characteristics, and it is assumed that all ecto-ATPases are E-type AT Pases. The converse is not true, however, since soluble E-type ATPases were shown to ex sist in plants, microorganisms, and the saliva of blood sucking insects. These enzymes could be easily purified, and exhibited very high specific activity.

Seller Inventory # 9781461377290

Bibliographic Details

• Title: Ecto-Atpases : Recent Progress on Structure and Function
• Publisher: Springer Us
• Publication Date: 2012
• Language: English
• ISBN 10: 1461377293
• ISBN 13: 9781461377290
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 254W x 178H x 17D millimeters 254 millimeters width by 178 millimeters height by 17 millimeters depth
• Weight: 590 grams

About this title

Synopsis

It has been known for almost 50 years that many cells carry enzymes that hydro- lyze extracellular ATP, and the term "ecto-ATPase" was used first by Engelhardt 40 years ago. But until the end of the 1970's, the idea of an ATPase with its ATP hydrolyzing site on the outside of the cell membrane was met with substantial skepticism since it was thought that ATP was strictly intracellular. Nevertheless, ecto-ATPase activity was dem- onstrated using a variety of intact cells. Most ecto-ATPase(s) exhibited three common 2 characteristics: 1) activation by either Ca + or Mg2+, 2) insensitivity to the commonly used inhibitors ofF-type, P-type, and V-type ATPases, and 3) ability to hydrolyze nucleo- side triphosphates and often nucleoside diphosphates as well. At the same time, the dominant ATPase activity in many plasma membrane preparations was shown to be dis- tinct from the ion-pump ATPases, but had similar enzymatic properties as the ecto-AT- Pase(s). Thus the term "E-type ATPase activity" has been proposed for ATPase activity exhibiting these characteristics, and it is assumed that all ecto-ATPases are E-type AT- Pases. The converse is not true, however, since soluble E-type ATPases were shown to ex- sist in plants, microorganisms, and the saliva of blood sucking insects. These enzymes could be easily purified, and exhibited very high specific activity.