Electrochemical synchronization of cell spreading on solid surfaces | Dictyostelium discoideum: synchronization of spreading

Marius Socol

ISBN 10: 3843360634 / ISBN 13: 9783843360630

Published by LAP LAMBERT Academic Publishing, 2010

About this Item

Electrochemical synchronization of cell spreading on solid surfaces | Dictyostelium discoideum: synchronization of spreading | Marius Socol | Taschenbuch | 168 S. | Englisch | 2010 | LAP LAMBERT Academic Publishing | EAN 9783843360630 | Verantwortliche Person für die EU: BoD - Books on Demand, In de Tarpen 42, 22848 Norderstedt, info[at]bod[dot]de | Anbieter: preigu.

Seller Inventory # 107273332

Bibliographic Details

• Title: Electrochemical synchronization of cell spreading on solid surfaces | Dictyostelium discoideum: synchronization of spreading
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2010
• Language: English
• ISBN 10: 3843360634
• ISBN 13: 9783843360630
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 220W x 150H x 11D millimeters 220 millimeters width by 150 millimeters height by 11 millimeters depth
• Weight: 268 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion. LimE- GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method.

About the Author

Marius Socol, PhD in Biophysics (2010) Grenoble Joseph Fourier University and PhD in Chemistry (2008) Cluj-Napoca Babe?-Bolyai University: studied Physics and Chemistry at Babe? Bolyai University of Cluj-Napoca. PostDoc research scientist at INSERM, U600, Luminy, Marseille.