Electrophoresis of Enzymes : Laboratory Methods

Gunter M. Rothe

ISBN 10: 3642790712 / ISBN 13: 9783642790713

Published by Springer Berlin Heidelberg, 2011

About this Item

Druck auf Anfrage Neuware - Printed after ordering - This book is addressed to both new and experienced workers interested in the properties of enzymes or using enzymes as gene markers in areas such as biochemi stry, clinical pathology, zoology, botany, systematics, ecology, population genetics or natural resources management. The first chapter introduces into the classification, structure and size of enzymes and isozymes. The second chapter provides protocols to extract (iso)enzymes from microbial, plant, animal and human tissue and cells-respectively. Methods to extract enzymes from cell-organelles of plant and animal tissues are also presented. The third chapter is dedicated to reliable electrophoretic procedures being in use to separate native enzymes. Some quantitative electrophoretic methods are also given to deter mine the size and net charge of (iso)enzymes. Chapter four summarizes methods to renature enzymes after sodium dodecylsulphate electrophoresis. Chapter five gives an overview on the various 'histochemical' reactions being in use to visualize enzymes following electrophoresis. Specific staining protocols together with suitable electrophoretic systems for more than 100 different enzymes are listed in chapter six. Finally, methods of data acquisition from enzyme patterns produced by electro phoresis are presented and various examples given to calculate populational genetic statistics. Acknowledgement I am very grateful to Prof. Dr. Bertold J. Radola who encouraged me to write this book. I am also much indebted to all my students but especially to Renate Bohrmann, Ute Does, Dr. Werner D. Maurer, Brigitte Niethard, Monika Noll, Dr. Irene Novotny, Ina Pappe, Dr. Huschang Pukhanbaba, Anke Richert and Beate Schellenberg.

Seller Inventory # 9783642790713

Bibliographic Details

• Title: Electrophoresis of Enzymes : Laboratory Methods
• Publisher: Springer Berlin Heidelberg
• Publication Date: 2011
• Language: English
• ISBN 10: 3642790712
• ISBN 13: 9783642790713
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 242W x 170H x 18D millimeters 242 millimeters width by 170 millimeters height by 18 millimeters depth
• Weight: 564 grams

About this title

Synopsis

The electrophoresis of enzymes is a well established technique in biochemical, clinical, environmental, microbiological and forensic laboratories. This book compiles hundreds of proven methods in detail. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every biochemistry laboratory.

Review

"...an invaluable practical guide for a wide application of classical'electrophoretic techniques" - The Analyst