This is an ex-library book and may have the usual library/used-book markings inside.This book has hardback covers. Book contains pencil markings. In poor condition, suitable as a reading copy. No dust jacket. Please note the Image in this listing is a stock photo and may not match the covers of the actual item,850grams, ISBN:9780199631421.

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Bibliographic Details

Title

Enzyme Assays: A Practical Approach (Practical Approach Series)

Publisher

IRL Press

Publication Date

1992

Language

English

ISBN 10

0199631425

ISBN 13

9780199631421

Binding

Hardcover

Condition

Poor

Weight

850 grams

Seller catalogs

Biology

About this title

Synopsis

Enzyme assays are one of the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific examples. Chapters contain experimental protocols, including photomeric, electrochemical, radiochemical, and HPLC techniques, along with methods for determining enzyme activity after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also discussed are the techniques and problems associated with enzyme extraction, statistical analysis of kinetic data, and a chapter examining buffers and methods of protein estimation. This work will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

About the Author

Michael J. Danson, both Senior Lecturers in Biochemistry, University of Bath.

"About this title" may belong to another edition of this title.

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