Enzyme Engineering (Hardcover)

Enzyme Engineering

An authoritative and up-to-date discussion of enzyme engineering and its applications

In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deliver a robust treatment of enzyme engineering and its applications in various fields such as biotechnology, life science, and synthesis. The book begins with an introduction to different protein engineering techniques, covers topics like gene mutagenesis methods for directed evolution and rational enzyme design. It includes industrial case studies of enzyme engineering with a focus on selectivity and activity.

The authors also discuss new and innovative areas in the field, involving machine learning and artificial intelligence. It offers several insightful perspectives on the future of this work.

Readers will also find:

• A thorough introduction to directed evolution and rational design as protein engineering techniques
• Comprehensive explorations of screening and selection techniques, gene mutagenesis methods in directed evolution, and guidelines for applying gene mutagenesis in organic chemistry, pharmaceutical applications, and biotechnology
• Practical discussions of protein engineering of enzyme robustness relevant to organic and pharmaceutical chemistry
• Treatments of artificial enzymes as promiscuous catalysts
• Various lessons learned from semi-rational and rational directed evolution

A transdisciplinary treatise, Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science is perfect for protein engineers, theoreticians, organic, and pharmaceutical chemists as well as transition metal researchers in catalysis and biotechnologists.

Manfred T. Reetz is Emeritus Professor at the Max-Planck-Institut für Kohlenforschung in Mülheim/Germany. He is a synthetic organic chemist who pioneered the concept of directed evolution of stereo- and regioselective enzymes as a prolific source of catalysts in organic chemistry and biotechnology.

Zhoutong Sun, PhD, is Full Professor at Tianjin Institute of Industrial Biotechnology at the Chinese Academy of Sciences. His research interests include enzyme engineering,metabolic engineering and synthetic biology.

Ge Qu, PhD, is Associated Professor at the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences/China and member of the Sun group. He specializes in computational enzyme design and biocatalysis.

Manfred T. Reetz is a former Director and presently Emeritus Professor at the Max-Planck-Institut für Kohlenforschung in Mülheim/Germany. He is a synthetic organic chemist who pioneered the concept of directed evolution of stereo- and regioselective enzymes as a prolific source of catalysts in organic chemistry and biotechnology. Reetz has received numerous German and international awards and prizes, and is member of the German National Academy Leopoldina, Academy of the German state of NRW, Royal Dutch Academy of Sciences, European Academy of Sciences, and Distinguished Scientist of the Chinese Academy of Sciences (China).

Zhoutong Sun obtained his Ph.D in microbiology at Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences in 2012, then he moved to Nanyang Technological University in Singapore as a research fellow. One year later, he moved to the MPI für Kohlenforschung and Marburg University for a postdoc with Prof. Manfred T. Reetz. In 2016, he became a full professor at Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences. His research interests are in the discovery, design and engineering of biocatalysts as well as cascade reactions design and metabolic engineering.

Ge Qu received his Ph.D in Bioinformatics from Adam Mickiewicz University in Poland in 2015. Currently, he is working as an associated professor in the Reetz Laboratory at Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences. His major research interests are the discovery and structure-based engineering of enzymes that have potential as industrial biocatalysts.