Establishing a Cell Biology Platform | Isolation and Preservation of Human Blood Products

Graça Marques (u. a.)

ISBN 10: 3659217441 / ISBN 13: 9783659217449

Published by LAP LAMBERT Academic Publishing, 2014

About this Item

Establishing a Cell Biology Platform | Isolation and Preservation of Human Blood Products | Graça Marques (u. a.) | Taschenbuch | 140 S. | Englisch | 2014 | LAP LAMBERT Academic Publishing | EAN 9783659217449 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.

Seller Inventory # 105363204

Bibliographic Details

• Title: Establishing a Cell Biology Platform | Isolation and Preservation of Human Blood Products
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2014
• Language: English
• ISBN 10: 3659217441
• ISBN 13: 9783659217449
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 220W x 150H x 9D millimeters 220 millimeters width by 150 millimeters height by 9 millimeters depth
• Weight: 227 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

The use of human primary cells provide researchers in different areas with irrefutable more biologically relevant data than using cell lines or animal blood cells. This work was performed in the scope of the Cell Biology Services @ CEDOC, a platform aiming to provide viable and trustful human primary cells and products. We had three main objectives: protocol optimizations for human blood cell isolation, culture and cryopreservation; cost estimation and divulgation of the services. In this book, we review standard protocols and compare different strategies for blood cell isolation (peripheral blood mononuclear cells, T cells and monocytes). The impact of those methodologies was evaluated regarding cell yield and purity, cell functional characteristics and cost. We have also developed a method for serum isolation from human plasma in blood buffy coats. Testing commercial products and optimizing protocols is very important to the implementation of methodologies and standardized practices for research purposes, either by the academic community or companies.

About the Author

Graça Marques completed her Master degree in Molecular Genetics and Biomedicine at NOVA Medical School in 2013, under the supervision of Zélia Silva and Paula VIdeira. Graça is presently a Research Fellow at the Glycoimmunology Group at CEDOC, FCM/UNL. She masters several immunological and cell biology techniques.