Evolutionary Methods in Biotechnology. Dirty Tricks for Directed Evolution: Clever Tricks for Directed Evolution : Dirty Tricks for Directed Evolution

Susanne Brakmann,Andreas Schwienhorst

ISBN 10: 3527307990 / ISBN 13: 9783527307999

Published by Wiley-VCH, 2004

About this Item

Neu Auf Lager, Sofortversand per DHL/dpd aus Deutschland - Wie erstellt und durchsucht man kombinatorische Enzymbibliotheken Dieser Band stellt Ihnen erstmals geprüfte, ausführliche Protokolle zur Verfügung. Die Autoren der Beiträge, Experten mit eigener Erfahrung in der evolutionären Biotechnologie, kommentieren die Arbeitsanleitungen mit Hinweisen zu häufigen Fehlern und deren Beseitigung. Unentbehrlich fürjeden, der auf diesem interdisziplinären Gebiet arbeitet.

Seller Inventory # INF1000021187

Bibliographic Details

• Title: Evolutionary Methods in Biotechnology. Dirty Tricks for Directed Evolution: Clever Tricks for Directed Evolution : Dirty Tricks for Directed Evolution
• Publisher: Wiley-VCH
• Publication Date: 2004
• Language: English
• ISBN 10: 3527307990
• ISBN 13: 9783527307999
• Binding: Gebundene Ausgabe
• Condition: Neu
• Dimensions: 248W x 176H x 17D millimeters 248 millimeters width by 176 millimeters height by 17 millimeters depth
• Weight: 572 grams

About this title

Synopsis

Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed.
From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems.
Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution.
As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms.
In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.

About the Author

Susanne Brakmann is head of the junior research group "Applied Molecular Evolution" at the University of Leipzig (Germany) and a Member of the Biotechnological-Biomedical Center of Leipzig. She studied Chemistry at the Technical University of Braunschweig where she received her diploma in 1988, moving afterwards to the University of Karlsruhe to work on her thesis under the supervision of Reinhold Tacke (Ph. D. 1991). She was postdoctoral fellow at the Max-Planck-Institute for Biophysical Chemistry in Gottingen where she worked with Manfred Eigen before she moved to Leipzig in 2001. She is interested in directed evolution as a tool for understanding and optimizing enzyme functions, focusing on nucleic acid polymerases and their biotechnological applications.

Andreas Schwienhorst is a group leader at the Institute of Microbiology and Genetics at the University of Gottingen (Germany). He was born in Warendorf and did his studies of Biochemistry and Prehistory in Munster and Gottingen. His thesis was carried out at the Max-Planck-Institute for Biophysical Chemistry under the supervision of Manfred Eigen. He was a visiting scientist at the Salk Institute, La Jolla and the Los Alamos National Laboratory (USA), then took a position as a group leader at the Institute for Molecular Biotechnology before moving to the University of Gottingen in 1997. In 1998 he received the Biofuture Award. Currently, he is interested in the discovery of novel targets for drug intervention as well as in methods of molecular evolution and their applications in drug discovery and catalysis.