Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.
Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following:

Baculovirus expression vectors
CHO cells
E. coli
HEK293-EBNA1 cells
Lactococcus lactis and other gram positive bacteria
S. cerevisiae
Transfected insect cells
Pichia pastoris
Mammalian cells using BacMam viruses
Lentiviral vectors
Wheat germ cell-free system

The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.
Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.
In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.

"About this title" may belong to another edition of this title.

Store Description

The Saint Bookstore has a range of over 1 million titles available.

Visit Seller's Storefront

Seller's business information

SB ONLINE LTD
50 Devonshire Road, Southport, PR9 7BZ, United Kingdom

Sale & Shipping Terms

Terms of Sale

Please order through the Abebooks checkout. We only take orders through Abebooks - We don't take direct orders by email or phone.

Refunds or Returns: A full refund of the purchase price will be given if returned within 30 days in undamaged condition.

As a seller on abebooks we adhere to the terms explained at http://www.abebooks.co.uk/docs/HelpCentral/buyerIndex.shtml - if you require further assistance please email us at orders@thesaintbookstore.co.uk

Shipping Terms

Most orders usually ship within 1-3 business days, but some can take up to 7 days.

Shipping rates from United Kingdom to U.S.A.

Shipping rates from United Kingdom to U.S.A.
Order quantity	7 to 28 business days	7 to 28 business days
First item	US$ 23.94	US$ 26.62

Delivery times are set by sellers and vary by carrier and location. Orders passing through Customs may face delays and buyers are responsible for any associated duties or fees. Sellers may contact you regarding additional charges to cover any increased costs to ship your items.