Expression Systems : Methods Express

Michael Dyson

ISBN 10: 1904842437 / ISBN 13: 9781904842439

Published by Scion Publishing Sep 2007, 2007

About this Item

Neuware - 'Expression Systems: Methods Express' is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice.

Seller Inventory # 9781904842439

Bibliographic Details

• Title: Expression Systems : Methods Express
• Publisher: Scion Publishing Sep 2007
• Publication Date: 2007
• Language: English
• ISBN 10: 1904842437
• ISBN 13: 9781904842439
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 244W x 171H x 15D millimeters 244 millimeters width by 171 millimeters height by 15 millimeters depth
• Weight: 680 grams

About this title

Synopsis

Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.
Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following:

• Baculovirus expression vectors
• CHO cells
• E. coli
• HEK293-EBNA1 cells
• Lactococcus lactis and other gram positive bacteria
• S. cerevisiae
• Transfected insect cells
• Pichia pastoris
• Mammalian cells using BacMam viruses
• Lentiviral vectors
• Wheat germ cell-free system

The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.
Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.
In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.