Expression of two insecticidal genes in Cotton | Development of Transgenic Cotton with sustained resistance against targeted insect pests

Allah Bakhsh (u. a.)

ISBN 10: 3844389202 / ISBN 13: 9783844389203

Published by LAP LAMBERT Academic Publishing, 2011

About this Item

Expression of two insecticidal genes in Cotton | Development of Transgenic Cotton with sustained resistance against targeted insect pests | Allah Bakhsh (u. a.) | Taschenbuch | 176 S. | Englisch | 2011 | LAP LAMBERT Academic Publishing | EAN 9783844389203 | Verantwortliche Person für die EU: BoD - Books on Demand, In de Tarpen 42, 22848 Norderstedt, info[at]bod[dot]de | Anbieter: preigu.

Seller Inventory # 106702741

Bibliographic Details

• Title: Expression of two insecticidal genes in Cotton | Development of Transgenic Cotton with sustained resistance against targeted insect pests
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2011
• Language: English
• ISBN 10: 3844389202
• ISBN 13: 9783844389203
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 220W x 150H x 11D millimeters 220 millimeters width by 150 millimeters height by 11 millimeters depth
• Weight: 280 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton.

About the Author

Dr. Allah Bakhsh was born in Khanewal,(Pakistan)on April 15,1981. He completed four years honor degree in Agricultural sciences majoring in Plant Breeding and Genetics from University of Agriculture, Faisalabad, Pakistan in 2004. Afterwards, he joined CEMB, University of the Punjab, Lahore, Pakistan and completed his PhD in Molecular Biology.