Flow Cytometry: First Principles

Flow Cytometry First Principles Alice Longobardi Givan Geared for the nonspecialist, Flow Cytometry: First Principles presents a succinct, accessible "user friendly" approach to exploring flow cytometry and its relevance to modern biology and biomedicine. Starting with the fundamentals of instrumentation, data analysis, and interpretation of experimental and diagnostic results, the author provides a tutorial treatment of applications both in the laboratory and the clinic that will appeal to the novice and expert alike. Using concrete examples to illustrate general concepts, this self-teaching guide builds the reader’s technical understanding of the capabilities and limitations of modern analytical cytology. Students, research faculty, clinical specialists, and laboratory technologists will gain an appreciation of the range of experimental procedures and diagnostic measurements that are possible with today’s flow cytometric instrumentation. An outgrowth of years of lecture and laboratory workshop instruction by the author, Flow Cytometry: First Principles is designed for the needs of those in cell and molecular biology, immunology, oncology, hematology, laboratory medicine, and other areas that rely upon the quantitative measurement and selective purification of cells and their constituents. This practical introduction will prove indispensable for the nonspecialist who wishes to understand the power and the pitfalls of this technology — toward a more critical interpretation of the literature as well as the effective design and implementation of laboratory protocols.

Examines flow cytometry and its applications in research and diagnostic laboratory settings. Coverage includes basic principles of instrumentation, sample preparation and analysis. Presents research techniques in the life and biomedical sciences along with diagnostic uses in analytical cytology.