Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. The advent of solid supports, new DNA labeling techniques, and PCR technology has created a need for an up-to-date practical manual in this area. Gene Probes (Applications), describes techniques for specific research goals. It covers: hybridization strategies; choosing probes; screening and analysis of DNA libraries, including the use of PCR; blotting techniques, gene targeting; and analysis of human genetic mutations. Its companion volume, Gene Probes 1, describes the wide rage of techniques now available for the purification, synthesis, labeling, detection, and quantitation of probes.

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S. J. Higgins is at University of Leeds.

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