Gene Essentiality

Long Jason Lu

ISBN 10: 1493948628 / ISBN 13: 9781493948628

Published by Springer New York Okt 2016, 2016

About this Item

This item is printed on demand - it takes 3-4 days longer - Neuware -This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study. 260 pp. Englisch.

Seller Inventory # 9781493948628

Bibliographic Details

• Title: Gene Essentiality
• Publisher: Springer New York Okt 2016
• Publication Date: 2016
• Language: English
• ISBN 10: 1493948628
• ISBN 13: 9781493948628
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 254W x 178H x 14D millimeters 254 millimeters width by 178 millimeters height by 14 millimeters depth
• Weight: 555 grams

About this title

Synopsis

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.