Gene Silencing by RNA Interference: Technology and Application

Muhammad Sohail, Graeme Doran, M. Barciszewska-Pacak, Olivier Donze, Frank Buchholz, Monica Bettencourt-Dias, Queta Boese, Dong Sung An, Peter J. Appleford, Anke Bruning, Natasha J. Caplen, Palakodeti V.N. Dasaradhi, Andrew J. Hollins, Earl Gordon, Hidetoshi Hasuwa, Kin Hang Kok, Erwei Song, Robert W. Willams, Edwin M. Southern, James C. Morris, Femke Simmer, Renu Wadhwa, Daniel Silhavy, Valentine M. Macaulay, Frederick Meins Jr., B. Grabowska, J. Wojciechowicz, A. Pacak, Marek Figlerowicz, Didier Picard, Peter Dudek, Ulrich Klahre, David Drechsel, Martine Ruer, Ralf Kittler, William S. Marshall, Geoffrey W. Abbott, Saghir Akhtar, Vincent C. Auyeung, David Baltimore, Virander S. Chauhan, Irving S. Y. Chen, Mark E. Drew, Paul T. Englund, Ste

ISBN 10: 0849321417 / ISBN 13: 9780849321412

Published by CRC Press 2004-08-27, 2004

About this Item

Seller Inventory # 6666-TNFPD-9780849321412

Bibliographic Details

• Title: Gene Silencing by RNA Interference: Technology and Application
• Publisher: CRC Press 2004-08-27
• Publication Date: 2004
• Language: English
• ISBN 10: 0849321417
• ISBN 13: 9780849321412
• Binding: Hardcover
• Condition: New

About this title

Synopsis

Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet. This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.

About the Author

Muhammad Sohail