Gene Targeting and Embryonic Stem Cells (Advanced Methods)

Mcwhir, Jim and Thomson, Alison

ISBN 10: 1859963609 / ISBN 13: 9781859963609

Published by Taylor and Francis, 2004

About this Item

Dispatched, from the UK, within 48 hours of ordering. Though second-hand, the book is still in very good shape. Minimal signs of usage may include very minor creasing on the cover or on the spine.

Seller Inventory # CHL6380749

Bibliographic Details

• Title: Gene Targeting and Embryonic Stem Cells (Advanced Methods)
• Publisher: Taylor and Francis
• Publication Date: 2004
• Language: English
• ISBN 10: 1859963609
• ISBN 13: 9781859963609
• Binding: Paperback
• Condition: Used; Very Good

About this title

Synopsis

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.

About the Author

Jim McWhir (The Roslin Institute, UK) (Author) ,  Alison Thomson (The Roslin Institute, UK) (Author)