Genetic Diversity Analysis of Trichoderma Isolates Using SSR Markers: Study of Genetic Diversity Using Molecular Markers of Trichoderma

Srivastava, Swapnil; Kumar, Mukesh; Shami, Vashali

ISBN 10: 6207639480 / ISBN 13: 9786207639489

Published by LAP LAMBERT Academic Publishing, 2024

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Bibliographic Details

• Title: Genetic Diversity Analysis of Trichoderma Isolates Using SSR Markers: Study of Genetic Diversity Using Molecular Markers of Trichoderma
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2024
• Language: English
• ISBN 10: 6207639480
• ISBN 13: 9786207639489
• Binding: Soft cover
• Condition: New

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Synopsis

Trichoderma is a genus of fungi in the family Hypocreaceae found in all types of soils and is the most common culturable fungus. They occur worldwide and can be easily isolated from soil, plant organic matter, decaying wood etc. It is a well-known aggressive biocontrol agent and has a higher potential for reproduction and colonisation. In this study the genetic diversity among fourteen Trichoderma isolates (TBT- 01 to TBT- 14) was evaluated using ten SSR markers. Rhizospheric soil samples from various locations of Uttar Pradesh were collected and subjected to serial dilution and plating on a Trichoderma Selective Media. The isolates’ colonies were pure cultured on Potato Dextrose Agar for DNA isolation and molecular studies. Ten SSR primers (SSR-01, SSR- 06, SSR- 08, SSR- 10, SSR- 11, SSR-13, SSR- 14, SSR- 15, SSR- 18, and SSR- 20) were used for the PCR amplification of the DNA isolated.