Title: Genetic Diversity Analysis of Trichoderma Isolates Using SSR Markers: Study of Genetic Diversity Using Molecular Markers of Trichoderma
Publisher: LAP LAMBERT Academic Publishing
Publication Date: 2024
Language: English
ISBN 10: 6207639480
ISBN 13: 9786207639489
Binding: Soft cover
Condition: New

About this title

Synopsis

Trichoderma is a genus of fungi in the family Hypocreaceae found in all types of soils and is the most common culturable fungus. They occur worldwide and can be easily isolated from soil, plant organic matter, decaying wood etc. It is a well-known aggressive biocontrol agent and has a higher potential for reproduction and colonisation. In this study the genetic diversity among fourteen Trichoderma isolates (TBT- 01 to TBT- 14) was evaluated using ten SSR markers. Rhizospheric soil samples from various locations of Uttar Pradesh were collected and subjected to serial dilution and plating on a Trichoderma Selective Media. The isolates’ colonies were pure cultured on Potato Dextrose Agar for DNA isolation and molecular studies. Ten SSR primers (SSR-01, SSR- 06, SSR- 08, SSR- 10, SSR- 11, SSR-13, SSR- 14, SSR- 15, SSR- 18, and SSR- 20) were used for the PCR amplification of the DNA isolated.

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