Genetic Engineering and Biotechnology : Concepts Methods and Applications

Contents Preface. 1. Mendel to molecules/R.P. Sharma and V.L. Chopra. 2. Biochemical and biophysical techniques used in recombinant DNA work/I.M. Santha K.R. Koundal and S.L. Mehta. 3. Purification and characterization of DNA RNA and proteins/S. Muthukrishnan. 4. cDNA synthesis nick translation and in vitro translation/Tapan K. Misra. 5. DNA sequencing and synthesis/Roland Brousseau Mary A Hefford and Peter C.K. Lau. 6. Transposon Tn5 Mutagenesis/James R. Lupski. 7. Biotechnology for crop improvement/V.L. Chopra and S.B. Narasimhulu. 8. Genetic engineering in medicine/James R. Lupski. Index. Genetic engineering provides a precise and directed method of modifying genetic material and the possibilities to control the processes of genetic expression and regulation. Coupled with cellular genetic manipulation genetic engineering has become a very potent tool of generating technologies which have a far reaching relevance to a diversity of objectives in many sectors. For the proper understanding of genetic engineering and biotechnological methods however an introduction to and appreciation of numerous biochemical and biophysical techniques is necessary. Equally important are the concepts on which these techniques and their applications are based. The book aims at providing the much needed information to a serious beginner on the philosophy techniques and methodologies of genetic engineering and biotechnology and their application to the two important areas of medicine and crop improvement. There are eight papers written by international experts and the treatment bears the seal of authenticity. The book will be of invaluable help to students researchers and all those interested in the principles and practice of genetic engineering and biotechnology. (jacket) 200 pp.…