Genetic Library Construction and Screening : Advanced Techniques and Applications (Lab Manuals Ser.).

R. C. (editor) and B. F. (editor) Smith Bird

ISBN 10: 3540672788 / ISBN 13: 9783540672784

Published by New York, NY, U.S.A. Springer-Verlag New York, Incorporated, 2002

About this Item

Gebraucht - Wie neu Remittende, About this book This volume is designed to provide an easily accessible introduction to core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies, such as variations on two-hybrid analysis for investigation of protein-protein interactions or investigation of protein-nucleic acid interactions. Written for scientists working in the disciplines of molecular biology and genetics, graduate students.

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Bibliographic Details

• Title: Genetic Library Construction and Screening : Advanced Techniques and Applications (Lab Manuals Ser.).
• Publisher: New York, NY, U.S.A. Springer-Verlag New York, Incorporated
• Publication Date: 2002
• Language: English
• ISBN 10: 3540672788
• ISBN 13: 9783540672784
• Binding: Softcover
• Condition: Wie neu

About this title

Synopsis

Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.