Genetic Transformation Systems in Fungi

Fungi are an economic very important class of microbes. Not only do they host a range of versatile enzymes used in industrial applications (biofuels, laundry, food processing), as well do they produce several very important pharmaceutical drugs (statins and penicillins). Moreover, fungal pathogens can cause great damage in agricultural production (Phytophthora and Botrytis) and during mammalian infections (Penicillium marneffei and Candida). Transformation of DNA is used to understand the genetic basis behind these traits. Several different techniques have been developed over the years and readily shown to be decisive methods to improve fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples to be used in laboratories worldwide.

Marco A. van den Berg, PhD

Principal Scientist Applied Biochemistry and Screening

DSM Biotechnology Center

Delft, The Netherlands

 Karunakaran Maruthachalam, PhD

Current: Research Scientist

Global Marker Technology Lab, DuPont-Pioneer

DuPont Knowledge Center

Andhra Pradesh, India

 Previous: Molecular Biologist

California Seed and Plant Labs

Pleasant Grove, CA, USA